Characterization of effects of endothelin-1 on the L-type Ca current in human atrial myocytes
نویسندگان
چکیده
CHRISTOPHE BOIXEL,1 SYLVIE DINANIAN,2 LOÎC LANG-LAZDUNSKI,3 JEAN-JACQUES MERCADIER,1,4 AND STEPHANE N. HATEM1,4 1Institut National de la Santé et de la Recherche Médicale U460, Faculté de Médecine Xavier Bichat, 75018 Paris; 2Service de Cardiologie Hôpital Antoine Béclère, 92141 Clamart; and 3Service de Chirurgie Cardiaque and 4Service de Physiologie-Explorations Fonctionnelles, Groupe Hospitalier Bichat-Claude Bernard, Assistance Publique-Höpitaux de Paris, 75018 Paris, France
منابع مشابه
Characterization of effects of endothelin-1 on the L-type Ca2+ current in human atrial myocytes.
The effects of endothelin-1 (ET-1) on the L-type Ca2+ current (I(Ca)) were examined in whole cell patch-clamped human atrial myocytes. Depending on the initial current density, ET-1 (10 nM) increased the amplitude of I(Ca) by 99 +/- 7% or decreased it by 33 +/- 2%. The stimulatory effect predominated on current of low density (2.3 +/- 0.2 pA/pF), whereas I(Ca) of higher density (5.8 +/- 0.3 pA/...
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Plasma endothelin-1 (ET-1) levels are elevated in spinal cord injury (SCI), and ET-1 may be involved in the pathophysiology of this condition. However, its effects on contractile function of the heart of SCI rats are still unknown. To define more clearly the possible role of ET-1 following SCI, we investigated the effect of ET-1 on the contraction, calcium transients and L-type calcium current ...
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The effects of tyrosine protein kinases (TK) on the L-type Ca(2+) current (I(Ca)) were examined in whole cell patch-clamped human atrial myocytes. The TK inhibitors genistein (50 microM), lavendustin A (50 microM), and tyrphostin 23 (50 microM) stimulated I(Ca) by 132 +/- 18% (P < 0.001), 116 +/- 18% (P < 0.05), and 60 +/- 6% (P < 0.001), respectively. After I(Ca) stimulation by genistein, exte...
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Endothelin-1 (ET-1) is elevated in patients with atrial fibrillation (AF) and heart failure. We investigated effects of ET-1 on human atrial cellular electrophysiological measurements expected to influence the genesis and maintenance of AF. Action potential characteristics and L-type Ca(2+) current (I(CaL)) were recorded by whole cell patch clamp, in atrial isolated myocytes obtained from patie...
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